CytoFLEX LX Flow Cytometer

The CytoFLEX LX Flow Cytometer is an expansion of the CytoFLEX Platform. This model expands research possibilities with up to six lasers and 21 color parameters. Six spatially separated lasers allows panels to be spread across the spectrum reducing cross talk and spectral overlap. All instruments in the platform utilize CytExpert for CytoFLEX Acquisition and Analysis Software.

  • Two series are available, UV-Violet-blue-Yellow Green-Red-IR (U-V-B-Y-R-I) and Near UV-Violet-Blue-Yellow Green-Red-IR (N-V-B-Y-R-I)
  • Instruments with as few as four activated laser and 14 color parameters are available with the option to activate additional lasers and detection channels in the future with the purchase of an activation key
  • For higher throughput applications an optional plate loader module is available and for use in regulated environments the software contains tools for 21 CFR Part 11 compliance

CytoFLEX LX Akış Sitometresi Özellikleri

Exquisite Sensitivity

Çok Yönlü Platform

Düşük Bakım

Automation Enabled

  • Optional 96-well plate sample loader
  • API (Application Programming Interface) allows external software to control the instrument
  • For automated sample processing and data acquisition integrate with the Biomek i-Series Instrument

Explore CytoFLEX LX Models

CytoFLEX LX Akış Sitometresi Spesifikasyonları

Detection Photodiodes
Resolution <300 nm particles (488 nm side scatter), <80 nm polystyrene particles (405 nm side scatter), rCV 3%
Performance <30 MESF for FITC, <10 MESF for PE
Flow Cell Patent-pending, alignment-free integrated optics quartz flow cell (420 μm x 180 μm ID) design with >1.3 numerical aperture
Signal Processing Digital system with 7-decade display
Power Requirements 100-240 VAC, 50/60 Hz
Item Specifications Referenced C00445

Content and Resources

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Flow-cytometry-application-note-13-color-panel-t-cell-subsets-whole-blood Contains a comprehensive overview of T cell biology and the implications for multicolor immunophenotyping panel design. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library.
Flow-cytometry-application-note-8-color-panel-unlysed-whole-blood This whole blood assay does not involve harsh lysis or centrifugation steps, minimizing any cell perturbations from sample handling. This 8-color panel provides a broad survey of peripheral blood leukocytes.
Flow-cytometry-application-note-natural-killer-cells-preeclampsia-model A special population of natural killer cells (uNK) has been implicated in preeclampsia. uNK cells have contact with the trophoblast, modify uterine arteries to promote blood flow, and modulate trophoblast invasion in a dose-dependent manner. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided.
Flow-cytometry-application-note-phagocytosis-of-bacteria-whole-blood Describes an opsonization assay which can be used to assess the phagocytic capabilities of immune cell in whole blood. The benefits of using multicolor flow cytometry for the assay is that different phagocytic potential across various immune cell types can be determined in the same sample.
Flow-cytometry-application-note-dna-binding-dyes-for-viability-assesment Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.

Technical Documents