Gallios Flow Cytometer

The Gallios Flow Cytometer provides efficient acquisition of up to 12 parameters with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was designed for researchers needs.

Automated walk-away processing
Wide dynamic range of fluorescence intensities
Available with Kaluza for Gallios acquisition software
Remote diagnostics minimize downtime

Gallios Flow Cytometer Features

System Configurations

2 laser (red and blue), 6 color system.
2 laser (red and blue), 8 color system
3 laser (red, blue, and violet), 10 color system
4 laser (red, blue, violet and yellow/green), 10 color system

Workflow

Easy setup of experiments and automated walk-away processing
QC functionality with Levey-Jennings reports with Gallios CXP software
Improved software user interface with Kaluza for Gallios Acquisition software.

Set up and acquisition

6 decades of sensitivity and resolution to distinguish a wide range of populations easily.
Vortex of individual tube prior to acquisition
Adjustable probe for precious samples
Scatter resolution: Resolves 0.404 µm diameter particles from background noise using forward scatter with maximum detection of up to 40 µm diameter particles

Kaluza for Gallios Acquisition Software

Designed for novice to expert users
Visual management tools to help you understand what is happening with your experiment and cytometer.
No user profiles or login require to run acquisition
Set up a 10 color experiment in seconds
Offline software for developing protocols
Simulator to view acquisition of .fcs files which is particularly valuable for training

Gallios Flow Cytometer Specifications

Detection	Forward scatter: Fourier design (up to 3 measurements of forward angle), Side scatter: independently-focused photodiode with electronic attenuation, Fluorescence: FL1-FL10
Flow Cell	150 x 460 µm rectangular quartz gel coupled with 1.2 NA lens
Signal Processing	Dynamic range: 20-bit date acquisition, 1,048,576-channel workstation resolution, Digital sampling rate: 40MHz, Digital accuracy: < 5% error

BioSafe	Yes
Power Requirements	50/60 Hz, 100-240 VAC
Item Specifications Referenced	A94299

Gallios Flow Cytometer Documentation

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Title	Description
Kaluza for Gallios Acquisition Software Sophisticated Flow Cytometry, Simplified. {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Kaluza for Gallios from Beckman Coulter employs cutting-edge technology to make high complexity flow cytometry easy for even the least experienced, while providing powerful tools for the most sophisticated user
Flow Cytometry — Powerful Versatile Performance {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Built on a platform designed with reliability and stability in mind, Gallios samples information at 40MHz and displays data on a 1,048,576 channel scale.
Flow Cytometric Analysis of Endothelial Progenitor Cells {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	Authors: Dorota Sadowicz, Vasilis Toxavidis, John Tigges \| Beth Israel Deaconess Medical Center \| Harvard Stem Cell Institute
Major Swiss Medical Research Center Increases Cell Sorting Capability {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	The Geneva Medical College has doubled its activities since 2010, and has an increasing number of reseachers
Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	Authors: William L. Godfrey, Li Yang, Samantha Bauchiero, James W. Tung, Valerie Mallet, Lidice Lopez, Vincent Shankey, Emmanuel Gautherot
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung
Epithelial Cell Death Analysis of Cell Cycle by Flow Cytometry {E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}	Authors: Savithri Balasubramanian, John Tigges, Vasilis Toxavidis and Heidi Mariani
Cell Signaling Assay - Hardware Integration on Beckman Coulter Biomek {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Valentin Quesada \| Kelechi Eluwa \| Lidice Lopez \| Vincent Shankey \| Robert Auer \| Sergei Gulnik
Beyond Matching Dim Expression Densities with Bright Fluorochromes A Novel Practical Approach to Design Multicolor Applications {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Author: Michael Kapinsky, Beckman Coulter Life Science, Inc., Krefeld, Germany
PerFix-nc (No Centrifuge Assay): A New Intracellular No-Wash Assay for Whole Blood Analysis {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Fabrice Malergue, Valerie Mallet, Laeticia Khemici, Caroline Scifo, Sylvain Monseaux, Tewfik Miloud, Emmanuel Gautherot, Felix A. Montero-Julian \| Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France
SWOFF - The Unrecognized Sibling of FMO {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Author: Michael Kapinsky - Beckman Coulter Intl.
Simple and Robust Whole Blood Staining Procedure using PerFix-nc Reagents Generates Unprecedented FOXP3 Signal/Noise Ratio {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Fabrice Malergue, Laeticia Khemici, Tewfik Miloud, Carlos A. Garcia, Félix A. Montero-Julian \| Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France, Life Science Research, Miami, USA
Designing Multi-Color Panels for T/Treg Populations using FITMaN Guidelines: a Case Study {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: James W Tung, Jonel Lawson, Carlos A Garcia, Jeffrey R Cobb, Yetty Irwan, William Godfrey, Michael Reed, Enrique Rabellino \| Beckman Coulter Inc, Cellular Analysis Business Group, Scientific Affairs, Miami FL
FoxP3+ Tregs Cells Sensitivity to Low Doses of IL-2 Can be Rapidly and Easily Monitored by p-STAT-5 Detection in Ex-Vivo Whole Blood Samples {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Fabrice Malergue, Valarie Mallet, Caroline Scifo, Tewfik Miloud, Felix A. Montero-Julian \| Beckman Coulter Life Sciences, Inc. ; Global Assay and Applications Development, Marseille, France
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung - Beckman Coulter, Miami FL
Flow Cytometric Analysis of Multiple Signaling Pathways in LPS Stimulated Human Peripheral Blood Monocytes {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: T Shankey,Sue Chow, and David Hedley \| Advanced Technology Group, Beckman Coulter, Inc., Miami, FL, USA , Division of Applied Molecular Oncology, Princess Margaret Hospital, Univ of Toronto, Toronto, Ont., Canada
Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: William L. Godfrey, Li Yang, Samantha Bauchiero, James W. Tung, Valerie Mallet, Lidice Lopez, Vincent Shankey, Emmanuel Gautherot \| Beckman Coulter Inc, Miami, FL, USA; Marseille, FRANCE
Krome Orange™: A Novel Orange-Emitting Dye for Use in Multicolor Flow Cytometry Panels Using Violet Diode Laser Excitation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Felix Montero, Ravinder Gupta, Hashem Akhavan-Tafti, Robert Eickholt, Mark Sandison, Rhonda Federspiel, Laura Nieto Gligorovsky, Franck Gaille, Jeffrey Cobb and Emmanuel Gautherot \| Beckman Coulter, Inc., Miami, FL, USA; Detroit, MI, USA; Marseille, FRANCE
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-Occurring Regulatory T-Cells {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Carlos A. Garcia Santana M.D., Ph.D., James W. Tung Ph.D. and Sergei Gulnik Ph.D. \| Life Science Research; Life Science Development, Beckman Coulter, Inc., Miami, FL., USA
An 8-color Panel for Detection of Human Blood Dendritic Cells by Flow Cytometry {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Author: Tewfik Miloud, Nathalie Dupas, Anders Kverneland, Mathias Streitz, Birgit Sawitzki and Felix A. Montero-Julian \| Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France , 2Institute of Medical Immunology, Charité Universitätsmedizin, Berlin, Germany Institute of Medical Immunology, Charité Universitätsmedizin, Berlin, Germany
Flow Cytometry Training {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Real-world training programs to maximize the value of your flow cytometer investment.
Gallios™ Quick Reference {B14306BD-BB46-4B27-BF3E-B11862085BC7}	The purpose of this Quick Reference is to provide a simple step by step outline of the information needed to perform various tasks on the system
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak \| Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc., Indianapolis, IN \| Published 2011

Gallios Flow Cytometer

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Gallios Flow Cytometer Features

System Configurations

Workflow

Set up and acquisition

Kaluza for Gallios Acquisition Software

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Gallios Flow Cytometer Specifications

Gallios Flow Cytometer Documentation

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