MoFlo Astrios Cell Sorter
Simplify your most complex sorting tasks with the Astrios EQ Series of high-speed flow cytometry sorters. The Astrios EQ and EQs deliver powerful sorting capability while reducing complexity.
- Available with up to 7 spatially separated lasers and 51 parameters
- Six-way jet-in-air proficiency
- Dual forward scatter (EQ) and single forward scatter (EQs) models
- Features [patent] enhanced Forward Scatter (eFSC) technology
Choose a MoFlo Astrios EQ Cell Sorter Model
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MoFlo Astrios EQ Cell Sorter Features
Simplify your most complex sorting tasks with the Astrios EQ Series of high-speed flow cytometry sorters. The Astrios EQ and EQs deliver powerful sorting capability while reducing complexity.
- Available with up to 7 spatially separated lasers and 51 parameters
- Six-way jet-in-air proficiency
- Dual forward scatter (EQ) and single forward scatter (EQs) models
- Features [patent] enhanced Forward Scatter (eFSC) technology
Optics
- 7 laser / 7 pinhole configurable system; 355 nm, 405 nm, 488 nm, 532 nm, 560 nm, 592 nm, 645 nm.
- Single Multi-Laser Shaping Optic (MLSO) for fiber-coupled lasers
- Flat-Top beam profile on fiber coupled lasers increases beam stability and alignment ease
- 7 lasers aligned through two alignment stages for quick sorter set up
Performance
- IntelliSort II: Beadless drop delay determination and monitoring
- 6-way sorting with a variety of collection devices (temperature control for all sort outputs available with water bath option)
- Reproducibly sort up to 1536-well plates (due to historically stable fluidics)
- Only sorter capable of 70,000 sort decisions/second validated performance
Electronics and Software
- Electronic capability to simultaneously detect 44 parameters
- > 100,000 events/second acquisition rate validated performance
- 1 billion events .fcs files with no parameter limit
- Up to 2 FSC channels including user-selectable FSC masks
- Optional additional SSC detection off each wavelength greater than or equal to 405 nm
Biosafety
- Custom Baker SterilGARD BSL2 cabinet
- Single turnkey aerosol evacuation direct from sorter sort chamber to HEPA enclosure
- Quick release nozzle design for easy access to nozzle tip
- CyClone plate arm with wiper seals back of sort chamber
MoFlo Astrios EQ Cell Sorter Specifications
| Detection | Up to 7 spatially separated lasers across 44 parameters acquired simultaneously (51 total available) , Dual Forward Scatter |
| Resolution | Resolves 0.2 μm diameter particles in scatter from background |
| Drop Dive Frequency | Adjustable up to 200kHz |
| Flow Cell | Jet-in-Air Nozzle: 70 μm, 100 μm |
| Signal Processing | Log, height, area, width & log area for each parameter |
| Power Requirements | USA 115 VAC; 50/60 Hz at 15 A, EU: 220 VAC; 50/60 Hz at 10, JP: 100 VAC; 50/60 Hz at 15 A |
| Item Specifications Referenced | B25982 |